Intravital microscopy identifies selectins that regulate T cell traffic into allografts
نویسندگان
چکیده
منابع مشابه
Intravital microscopy identifies selectins that regulate T cell traffic into allografts.
T cell homing to sites of injury and inflammation is a critical step for adaptive immune responses. While much has been learned regarding T cell homing to lymphoid tissues, few studies have directly observed trafficking events during an effector response. In this study, we developed a model that uses intravital fluorescence videomicroscopy to determine the molecules critical to T cell rolling w...
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The ability of T and B lymphocytes to migrate into skin allografts undergoing rejection was studied in mice. Spleen cells from CBA/J mice sensitized to transplantation antigens of A/J or C57BL/6 mice were separated on immunabsorbent columns into purified populations of T and B cells, labeled in vitro with 3H-uridine and injected intravenously into CBA/J mice with 7-day old skin iso and allograf...
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Natural killer T (NKT) cells were first recognized more than two decades ago as a separate and distinct lymphocyte lineage that modulates an expansive range of immune responses. As innate immune cells, NKT cells are activated early during inflammation and infection, and can subsequently stimulate or suppress the ensuing immune response. As a result, researchers hope to harness the immunomodulat...
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Intravital microscopy is an extremely powerful tool that enables imaging several biological processes in live animals. Recently, the ability to image subcellular structures in several organs combined with the development of sophisticated genetic tools has made possible extending this approach to investigate several aspects of cell biology. Here we provide a general overview of intravital micros...
متن کاملIntravital microscopy
In principle, there is little difference between conventional microscopy methods and intravital microscopy; in practice, however, there are significant differences in the way images are acquired and processed and a number of unusual features are associated with the microscope mechanics and other accessories. This note describes the approaches that we have developed over the last few years at GC...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 2003
ISSN: 0021-9738
DOI: 10.1172/jci19391